Composite

Part:BBa_K3715072:Design

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-27)


T7 promoter+RBS+ His+Linker f+SUMO+Linker d+PETase_MT14+Linker e+T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 296
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 296
    Illegal NheI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 296
    Illegal BglII site found at 185
    Illegal BamHI site found at 1285
    Illegal XhoI site found at 384
    Illegal XhoI site found at 1271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 296
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 296
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli BL21.



Source

NCBI


References